What are NK Cells?
NK cells are the first line of defense against infection, cancer and autoimmune disease. NK cells destroy their target by releasing toxic substances, perforin and granzymes, into the infected or abnormal cell resulting in target cell death
Engineered NK cell therapies overcome the limitations of T cell therapy for cancer
Improved solid tumor activity
Genome Editing Technique
CRISPR/Cas9 has recently emerged as a powerful technique for genetic modification. This technology relies on the introduction of Cas9 protein in conjunction with guide RNA into the NK cells. More recently, CRISPR/Cas9 has also been applied to introduce new genes. In parallel to CRISPR/Cas9, a homologous donor DNA template is introduced in the same cell via transfection.This DNA template replaces the targeted gene, thus allowing for the introduction of genes that promote the anti-tumor effects. When the CAR gene is delivered via viral vectors or using transposon-based random insertion, expression levels are variable. CAR expression driven by an endogenous promotor following CRISPR/Cas9-specific locus knock-in strategies could result in long CAR-T persistence in vivo, with a memory-like phenotype and less expression of exhaustion markers.Thus, the CRISPR/Cas9 strategy is a versatile technique that should be further explored for the generation of CAR-NK cells. It can be used to precisely delete, repair or introduce genes in a specific locus, hence holding promise to generate powerful anti-tumor NK cells.
OUR VIRAL VECTOR PLATFORM
Platform to support broad range of virus types. GMP production services using both transient transfection and stable packaging cell-line-based processes.Support production of vectors with either adherent or suspension-based platforms.
State of the Art Facility
Robust and scalable process strategies
GMP Cell Banking and Viral Seed stocks
Ability to handle different transduction processes
Analytical Lab handling impurity characterization and Higher Order Structures
Removing batch-to-batch variability and leading to a consistent, high-quality product.
Streamlining manufacturing to greatly increase dose capacity and reducing manufacturing costs