CREATING AN IMMORTALIZED CELL LINE FROM KIDNEY CELLS

In 1973, Frank Graham (a postdoc in Alex van der Eb’s group in the Netherlands) cultured cells from human embryonic kidneys (HEK) to generate an immortalized cell line by transfecting DNA from adenovirus type 5. By integrating into the genome of the cells, the adenovirus DNA should prevent the arrest of the cell cycle and therefore allow for continuous propagation of the resulting cell line.

Know more